Thirty-nine female with SLE meeting the American College of Rheumatology 1997 criteria for SLE, aged 16-47 years, SLEDAI score >5, disease duration 2 months-96 months, average consumption of steroids (Methylprednisolone <50 mg/day), and use at least one type of immunosupresant agent were studied after giving informed consent. SLE patients who are pregnant or breast-feeding, anemia, cancer, impaired kidney function, using hormone replacement therapy, consume folic acid and vitamin B supplements, anticonvulsants agent, carbamazepine, phenytoin, xanthopterin, tamoxifen, and theophylline were excluded from the study. Subjects were obtained from Rheumatology Clinic of Dr. Saiful Anwar Hospital, Malang, Indonesia. Twelve healthy, age matched premenopausal women (medical students, resident, and healthy blood donors from the same geographical area) were studied as a control group after giving informed consent. This study used an observational analytic cross sectional approach and it was conducted in .
5 cc out of venous bloodstream trials was indeed gathered into the test-tube that have aple try centrifuged at 3000 rpm for 5 min. The newest reste no indication of hemolysis are kept in tubes from the temperatures from -70°C for further analysis.
Individuals laboratory details and additionally gel homocysteine, folic acid, vitamin B6, supplement B12, bCTx, osteocalcin, and you can RANKL had been measured because of the enzyme-linked immunosorbent assay (ELISA) strategy. The next parameters was counted by the commercial ELISA Package: homocysteine (NovaTeinBio Individual ELISA System Full Hcy), folic acid (into the B6 (in the B12 (Habite B12 NovaTeinBio Human ELISA Kit), bCTx (Cusabio bCTx Peoples ELISA Equipment), osteocalcin (NovaTeinBio Person ELISA Kit Osteocalcin), and RANKL (NovaTeinBio Peoples ELISA Equipment RANKL). The degree of MDA are measured by the Thiobarbituric Acidic Reactive Material (TBARS) method.
Mathematical investigation is actually performed by the SPSS to have Window type 16. Mathematical details were claimed when it comes to mean and you can fundamental deviation. Contained in this studies, variables exhibiting p-value below 0.05 were said to be statistically tall correspondingly. Pearson correlation attempt was utilized to check the fresh correlation. Fortsätt läsa But a minor dating was receive ranging from gel homocysteine and you will nutritional B12 (p=0